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Single-Cell Adhesion Force Mapping of a Highly Sticky Bacterium in Liquid

preprint
submitted on 17.03.2021, 01:50 and posted on 18.03.2021, 04:42 by Satoshi Ishii, Shogo Yoshimoto, Katsutoshi Hori

The highly sticky bacterium Acinetobacter sp. Tol 5 adheres to various material surfaces via its cell surface nanofiber protein, AtaA. This adhesiveness has only been evaluated based on the amount of cells adhering to a surface. In this study, the adhesion force mapping of a single Tol 5 cell in liquid using the quantitative imaging mode of atomic force microscopy (AFM) revealed that the strong adhesion of Tol 5 was several nanonewtons, which was outstanding compared with other adhesive bacteria. The adhesion force of a cell became stronger with the increase in AtaA molecules present on the cell surface. Many fibers of peritrichate AtaA molecules simultaneously interact with a surface, strongly attaching the cell to the surface. The adhesion force of a Tol 5 cell was drastically reduced in the presence of 1% casamino acids but not in deionized water (DW), although both liquids decrease the adhesiveness of Tol 5 cells, suggesting that DW and casamino acids inhibit the cell approaching step and the subsequent direct interaction step of AtaA with surfaces, respectively. Heterologous production of AtaA provided non-adhesive Acinetobacter baylyi ADP1 cells with a strong adhesion force to AFM tip surfaces of silicon and gold.

History

Email Address of Submitting Author

khori@chembio.nagoya-u.ac.jp

Institution

Nagoya University

Country

Japan

ORCID For Submitting Author

0000-0003-1994-3124

Declaration of Conflict of Interest

No conflict of interest

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