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Silicon-Vacancy Nanodiamonds as High Performance Near-Infrared Emitters for Live-Cell Dual-Color Imaging

preprint
revised on 02.02.2021, 12:32 and posted on 03.02.2021, 08:07 by Weina Liu, Yan Liu, Viatcheslav N. Agafonov, Haoyuan Qi, Kaloian Koynov, Valery A. Davydov, Rustem Uzbekov, Ute Kaiser, Theo Lasser, Fedor Jelezko, Tanja Weil
Intracellular imaging is limited by a short bleaching time of fluorescent molecules and particles. In our work we used nanodiamonds with silicon-vacancy centers (SiV) obtained by high-pressure high-temperature synthesis based on metal-catalyst-free growth. They are coated with a polypeptide biopolymer that allows efficient cellular uptake. Our results demonstrate that high photostability and narrow emission in the near-infrared region of nanodiamonds with SiV allow live-cell dual-color imaging and intracellular tracking. Such a system has broad potential applications, which is not limited to live-cell bioimaging, but also include diagnostic (SiV as a nanosized thermometer) and theranostic (nanodiamonds as drug carrier).

Funding

SPP 1923:  Innate Sensing and Restriction of Retroviruses

Deutsche Forschungsgemeinschaft

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SFB 1279:  Exploiting the Human Peptidome for Novel Antimicrobial and Anticancer Agents

Deutsche Forschungsgemeinschaft

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ERC Synergy Grant 319130 - BioQ

Russian Foundation for Basic Research 18-03-00936

History

Email Address of Submitting Author

tweil@mpip-mainz.mpg.de

Institution

Max Planck Institute for Polymer Research

Country

Germany

ORCID For Submitting Author

0000-0002-5906-7205

Declaration of Conflict of Interest

There is no conflict of interest

Version Notes

Version 2.0

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