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Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

preprint
submitted on 20.07.2020 and posted on 21.07.2020 by Brae Petersen, Luke Gallion, Nancy Allbritton
Capillary electrophoresis (CE) is a highly efficient separation method capable of handling small sample volumes (~pL) and low (~yoctomole) detection limits, and as such is ideal for applications that require high sensitivity such as single-cell analysis. Low-cost CE instrumentation is quickly expanding but low-cost, open-source fluorescence detectors with ultra-sensitive detection limits are lacking. Silicon photomultipliers (SiPM) are inexpensive, low-footprint detectors with the potential to fill the role as a detector when cost, size, and customization are important. In this work we demonstrate the use of a SiPM in CE with zeptomolar detection limits and a dynamic range spanning five orders of magnitude, comparable to photomultiplier detectors. We characterize the performance of the SiPM as a highly sensitive detector by measuring enzyme activity in single cells. This simple, small footprint, and low-cost (<$130) light detection circuit will be beneficial for open-source, portable, and budget friendly instrumentation requiring high sensitivity.

Funding

MICROFABRICATED INSTRUMENTATION TO MEASURE SPHINGOLIPID SIGNALING IN HUMAN ACUTE MYELOID LEUKEMIA

National Cancer Institute

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A combined single cell gene expression and enzyme activity assay to study chemotherapy resistance in pancreatic ductal adenocarcinoma

National Cancer Institute

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Development of an Ultrasensitive Microdevice for the Investigation of Sphingosine Kinase Activity in Single Cells

National Cancer Institute

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History

Email Address of Submitting Author

bpeter2@uw.edu

Institution

University of Washington

Country

United States

ORCID For Submitting Author

0000-0001-9294-4487

Declaration of Conflict of Interest

No conflict of interest.

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