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Self-Assembled Fluid Phase Floating Membranes with Tuneable Water Interlayers

preprint
submitted on 21.06.2019 and posted on 24.06.2019 by Luke Clifton, Nicoló Paracini, Arwel V. Hughes, Jeremy H. Lakey, Nina-Juliane Seinke, Joshaniel F. K. Cooper, Martynas Gavutis, Maximilian W. A. Skoda

We present a reliable method for the fabrication of fluid phase unsaturated bilayers which are readily self-assembled on charged self-assembled monolayer (SAM) surfaces producing high coverage floating supported bilayers where the membrane to surface distance could be controlled with nanometer precision. Vesicle fusion was used to deposit the bilayers onto anionic SAM coated surfaces. Upon assembly the bilayer to SAM solution interlayer thickness was 7-10 Å with evidence suggesting that this layer was present due to SAM hydration repulsion of the bilayer from the surface. This distance could be increased using low concentrations of salts which caused the interlayer thickness to enlarge to ~33 Å. Reducing the salt concentration resulted in a return to a shorter bilayer to surface distance. These accessible and controllable membrane models are well suited to a range of potential applications in biophysical studies, bio-sensors and Nano-technology.


History

Email Address of Submitting Author

luke.clifton@stfc.ac.uk

Institution

ISIS Pulsed Neutron and Muon Source, Rutherford Appleton Laboratory

Country

United Kingdom

ORCID For Submitting Author

0000-0001-8754-362X

Declaration of Conflict of Interest

The authors declare no conflict of interest

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