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Bhoge_Saraogi 2020.pdf (13.7 MB)
Selective Functionalization at N2-Position of Guanine in Oligonucleotides via Reductive Amination
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submitted on 19.06.2020 and posted on 22.06.2020by Bapurao Bhoge, Ishu Saraogi
Chemo- and site-specific modifications in oligonucleotides have wide applicability as mechanistic probes in chemical biology. Here we have employed a classical reaction in organic chemistry, reductive amination, to selectively functionalize the N2-amine of guanine/2’-deoxyguanine monophosphate. This method specifically modifies guanine in several tested DNA oligonucleotides, while leaving the other bases unaffected. Using this approach, we have successfully incorporated desired handles chemoselectively into DNA oligomers.