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COVID19_Paper_DurdagiLab_26April_2020.pdf (9.01 MB)

Screening of Clinically Approved and Investigation Drugs as Potential Inhibitors of SARS-CoV-2 Main Protease and Spike Receptor-Binding Domain Bound with ACE2 COVID19 Target Proteins: A Virtual Drug Repurposing Study

preprint
revised on 26.04.2020, 07:40 and posted on 27.04.2020, 12:37 by Serdar Durdagi, Busecan Aksoydan, Berna Dogan, Kader Sahin, Aida Shahraki, Necla Birgül-İyison
In this virtual drug repurposing study, we used 7922 FDA approved drugs and compounds in clinical investigation from NPC database. Both apo and holo forms of SARS-CoV-2 Main Protease as well as Spike Protein/ACE2 were used for virtual screening. Initially, docking was performed for these compounds at target binding sites. The compounds were then sorted according to their docking scores which represent binding energies. The first 100 compounds from each docking simulations were initially subjected to short (10 ns) MD simulations (in total 300 ligand-bound complexes), and average binding energies during MD simulations were calculated using the MM/GBSA method. Then, the selected promising hit compounds based on average MM/GBSA scores were used in long (100-ns and 500-ns) MD simulations. In total around 15 µs MD simulations were performed in this study. Both docking and MD simulations binding free energy calculations showed that holo form of the target protein is more appropriate choice for virtual drug screening studies. These numerical calculations have shown that the following 8 compounds can be considered as SARS-CoV-2 Main Protease inhibitors: Pimelautide, Rotigaptide, Telinavir, Ritonavir, Pinokalant, Terlakiren, Cefotiam and Cefpiramide. In addition, following 5 compounds were identified as potential SARS-CoV-2 ACE-2/Spike protein domain inhibitors: Denopamine, Bometolol, Naminterol, Rotigaptide and Benzquercin. These compounds can be clinically tested and if the simulation results validated, they may be considered to be used as treatment for COVID-19.

History

Email Address of Submitting Author

serdar.durdagi@med.bau.edu.tr

Institution

Bahcesehir University, School of Medicine, Department of Biophysics

Country

Turkey

ORCID For Submitting Author

0000-0002-0426-0905

Declaration of Conflict of Interest

There is no conflict of interest

Version Notes

In this new version, top-30 docking poses used in short MD simulations were increased to top-100 docking poses, in addition, together with Main Protease target, we also used Spike Protein/ACE-2 complex for virtual screening. Same screening protocol of database was applied to Spike Protein/ACE-2 domain. Moreover, for selected hits at Main Protease, 500-ns MD simulations were performed. Trajectories of a few long MD simulations are also shared with the public at: http://durdagilab.com/?s=content&id=33

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