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SP3-FAIMS Chemoproteomics for High Coverage Profiling of the Human Cysteinome

preprint
submitted on 24.12.2020, 05:36 and posted on 28.12.2020, 06:40 by Tianyang Yan, Heta Desai, Lisa Boatner, Stephanie Yen, Jian Cao, Maria Palafox, Yasaman Jami-Alahmadi, Keriann Backus

We report a new cysteine chemoproteomic method, termed SP3-FAIMS chemoproteomics, which enables rapid and high coverage analysis of the human cysteinome. By combining enhanced cysteine biotinylation with SP3 sample decontamination and FAIMS online fraction, we identified in aggregate 34,225 unique cysteines found on 7,243 proteins. Showcasing the versatility of our method, integration with the isoTOP-ABPP workflow enabled the high throughput discovery of cysteines labelled by electrophilic compounds.

Funding

Beckman Young Investigator Award

V Scholar Award V2019-017

TRDRP T31DT1800

Chemistry Biology Interface Training Program T32GM008496

NIGMS System and Integrative Biology 5T32GM008185-33

History

Email Address of Submitting Author

kbackus@mednet.ucla.edu

Institution

UCLA

Country

United States

ORCID For Submitting Author

0000-0001-8541-1404

Declaration of Conflict of Interest

No conflicts of interest

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