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Reorganization of self-assembled DNA-based polymers using orthogonally addressable building blocks.pdf (4.22 MB)

Reorganization of Self-Assembled DNA-Based Polymers Using Orthogonally Addressable Building Blocks

submitted on 26.01.2021, 15:59 and posted on 27.01.2021, 12:50 by Serena Gentile, Erica Del Grosso, Leonard J. Prins, Francesco Ricci
Taking advantage of the addressability and programmability of DNA/DNA non-covalent interactions we report here the rational design of orthogonal DNA-based addressable tiles that self-assemble into polymer-like structures that can be reconfigured and reorganized by external inputs. The different tiles share the same 5-nucleotide sticky ends responsible for self-assembly but are rationally designed to contain a specific regulator-binding domain that can be orthogonally targeted by different DNA regulator strands (activators and inhibitors). We show that by sequentially adding specific activators and inhibitors it is possible to re-organize in a dynamic and reversible way the formed polymer-like structures to display well-defined distributions: homopolymers made of a single tile, random polymers in which different tiles are distributed randomly and block structures in which the tiles are organized in segments.


Email Address of Submitting Author


University of Rome Tor Vergata



ORCID For Submitting Author


Declaration of Conflict of Interest

No conflict of interest

Version Notes

Version 26/01/2021