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Regulating transition metal catalysis through interference by short RNAs copy.pdf (2.86 MB)
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Regulating Transition Metal Catalysis Through Interference by Short RNAs

preprint
submitted on 11.03.2019 and posted on 12.03.2019 by Sydnee Green, Hayden Montgomery, Tyler R. Benton, Neil Chan, Hosea Nelson
Here we report the discovery of a Au(I)-DNA hybrid catalyst that is compatible with biological media and whose reactivity can be regulated by small complementary nucleic acid sequences. The development of this catalytic system was enabled by the discovery of a novel Au(I) metal-mediated base pair. We find that Au(I) binds selectively to double stranded DNA containing C–T mismatches. In the Au(I)-DNA catalyst's latent state, the Au(I) ion is sequestered by the mismatch such that it is coordinatively saturated, rendering it catalytically inactive. Upon addition of an RNA or DNA strand that is complementary to the latent catalyst's oligonucleotide backbone, catalytic activity is induced leading to a 7-fold increase in formation of fluorescent product, forged through a Au(I)-catalyzed hydroamination reaction. Further development of this catalytic system will allow for temporal and spatial control of transition metal catalysis through gene transcription.

Funding

Pew Charitable Trust, David and Lucile Packard Foundation

History

Email Address of Submitting Author

sydneeagreen@ucla.edu

Institution

University of California, Los Angeles

Country

USA

ORCID For Submitting Author

0000-0003-3906-6616

Declaration of Conflict of Interest

no conflict of interest

Exports