Rational Enzyme Design Without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Glycosylation Catalysts
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We present an enzyme engineering approach based solely on amino-acids sequence to convert glycoside hydrolases into transglycosylases. We demonstrate its effectiveness on enzymes form five different glycoside hydrolase families, synthesizing various oligosaccharides containing different α-/β-pyranosides or furanosides in one-step with high yields.