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20200323__GHxx__ChemRxiv.pdf (3.49 MB)

Rational Enzyme Design Without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Glycosylation Catalysts

preprint
revised on 24.03.2020, 12:22 and posted on 24.03.2020, 13:21 by David Teze, Zhao, Jiao, Mathias Wiemann, Zubaida Gulshan Kazi, Rossana Lupo, Mette Errebo Rønne, Göran Carlström, Jens Øllgaard Duus, Yves-Henri Sanejouand, Michael J. O’Donohue, Eva Nordberg Karlsson, Régis Fauré, Henrik Stålbrand, Birte Svensson

We present an enzyme engineering approach based solely on amino-acids sequence to convert glycoside hydrolases into transglycosylases. We demonstrate its effectiveness on enzymes form five different glycoside hydrolase families, synthesizing various oligosaccharides containing different α-/β-pyranosides or furanosides in one-step with high yields.

Funding

NNF17OC0025660

History

Email Address of Submitting Author

david.teze@gmail.com

Institution

Technical University of Denmark

Country

Denmark

ORCID For Submitting Author

0000-0002-6865-6108

Declaration of Conflict of Interest

The authors declare no conflict of interest.

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