ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
1/1
0/0

Rapid Profiling of Isomeric Unsaturated Lipids Through Online Photochemical Derivatization of C=C Bonds

preprint
submitted on 07.09.2020 and posted on 10.09.2020 by Daisy Unsihuay, Pei Su, Yingju Li, Xiaofei Sun, Sudhansu Dey, Julia Laskin, Shihuan Kuang, Jiamin Qiu
Unraveling the complexity of the lipidome requires the development of novel approaches for the structural characterization of lipid species with isomeric resolution. Herein, we introduce an online photochemical approach for lipid isomer identification through selective derivatization of double bonds by reaction with singlet oxygen. Lipid hydroperoxide products are generated promptly after laser irradiation. Fragmentation of these species in a mass spectrometer produces diagnostic fragments, which reveal the C=C locations in the unreacted lipids. This approach uses an inexpensive light source and photosensitizer making it easy to incorporate into any lipidomics workflow. We demonstrate the utility of this approach for the shotgun profiling of C=C locations in different lipid classes present in tissue extracts using electrospray ionization (ESI) and for spatially-resolved analysis of lipids in tissue sections using nanospray desorption electrospray ionization (nano-DESI). These results provide a path for both rapid profiling and ambient imaging of positional isomers in biological samples.

Funding

NSF-1808136

HD068524

HD103475

PTEN Function in Satellite Cells

National Institute of Arthritis and Musculoskeletal and Skin Diseases

Find out more...

History

Email Address of Submitting Author

jlaskin@purdue.edu

Institution

Purdue University

Country

United States

ORCID For Submitting Author

0000-0002-4533-9644

Declaration of Conflict of Interest

no conflict of interest

Exports