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Rapid Intrabacterial Hydrolysis for Activating Antibiotics against Gram-negative Bacteria

submitted on 04.03.2019, 22:12 and posted on 05.03.2019, 21:04 by Jiaqing Wang, Deani L. Cooper, Wenjun Zhan, Difei Wu, Hongjian He, Shenghuan Sun, Susan T. Lovett, Bing Xu
Antimicrobial drug resistance demands novel approaches for improving the efficacy of antibiotics, especially against Gram-negative bacteria. Here we report that conjugating a diglycine (GG) to a prodrug of antibiotics drastically accelerates intrabacterial hydrolysis of ester bond for regenerating the antibiotics against E. coli. Specifically, the attachment of GG to chloramphenicol succinate (CLsu) generates a novel conjugate (CLsuGG), which exhibits about an order of magnitude higher inhibitory efficacy than CLsu against E. coli. This work, for the first time, illustrates that dipeptide conjugation modulates intrabacterial hydrolysis for increasing antibiotic efficacy and reducing adverse drug effects.


Email Address of Submitting Author


Brandeis University



ORCID For Submitting Author


Declaration of Conflict of Interest

no conflict of interest


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