These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.

Quantitative Characterization of Molecular-Stream Separation

revised on 22.07.2019 and posted on 22.07.2019 by Sven Kochmann, Sergey N. Krylov
Molecular-stream separation (MSS), e.g. by free flow electrophoresis or continuous annular chromatography, has great potential for applications that require continuous downstream separation such continuous flow synthesis. Despite its potential, MSS still needs to be greatly advanced, which requires currently lacking tools for quantitative characterization of streams in MSS. We developed and introduce here an analytical toolbox for this task. The first tool is a method to convolute 3D raw MSS data into a 2D “angulagram” via signal integration over the whole separation zone using a polar coordinate system. The second tool is three quantitative parameters characterizing stream width, linearity, and deflection, which are determined from an angulagram. The third tool is the analysis of the three parameters in relation to physicochemical characteristics of MSS which reveals deficiencies and guides improvements in MSS devices and methods. Examples of toolbox application to validation of previously published MSS data are provided.


Strategic Projects Grant 463455 from NSERC Canada


Email Address of Submitting Author


York University, Toronto



ORCID For Submitting Author


Declaration of Conflict of Interest

no conflict of interest

Version Notes

This version now includes a thumbnail with the specs given by Marshall Brennan (