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Pyclen Based Ln(III) Complexes as Highly Luminescent Bioprobes for in Vitro and in Vivo 1P and 2P Bioimaging Applications

preprint
submitted on 30.03.2020 and posted on 01.04.2020 by Nadège Hamon, Amandine Roux, Maryline Beyler, Jean-Christophe Mulatier, Chantal Andraud, Christophe Nguyen, Marie Maynadier, Nadir Bettache, Alain Duperray, Alexei Grichine, Sophie Brasselet, Magali Gary-Bobo, Olivier MAURY, Raphael Tripier
Two pyclen based lanthanide chelators, L4b and L4c, bearing two specific picolinate 2P antennas (tailor-made for each targeted metal) and one acetate arm arranged in a dissymmetrical manner, have been synthesized to form, with the already described ligand L4a, a complete family of lanthanide luminescent bioprobes: [EuL4a], [SmL4a], [YbL4b], [TbL4c] and [DyL4c]. Additionally, symmetrically arranged regioisomer L4a’ was also synthesized as well as its [EuL4a’] complex to highlight the astonishing positive impact of the dissymmetrical N-distribution of the functional chelating arm. The investigation clearly shows the high performance of each bioprobe, which, depending on the complexed lanthanide, could be used in various applications. Each presents high brightness, quantum yields and lifetimes. Staining of the complexes into living human breast cancer cells was observed. In addition, in vivo 2P-microscopy was performed for the first time on a living Zebrafish model with [EuL4a]. No apparent toxicity was detected on the growth of the zebrafish and images of high quality were obtained.

History

Email Address of Submitting Author

raphael.tripier@univ-brest.fr

Institution

University of Brest

Country

France

ORCID For Submitting Author

0000-0001-9364-788X

Declaration of Conflict of Interest

No Conflict of Interest

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