These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
Preprints are manuscripts made publicly available before they have been submitted for formal peer review and publication. They might contain new research findings or data. Preprints can be a draft or final version of an author's research but must not have been accepted for publication at the time of submission.
submitted on 30.03.2020 and posted on 01.04.2020by Nadège Hamon, Amandine Roux, Maryline Beyler, Jean-Christophe Mulatier, Chantal Andraud, Christophe Nguyen, Marie Maynadier, Nadir Bettache, Alain Duperray, Alexei Grichine, Sophie Brasselet, Magali Gary-Bobo, Olivier MAURY, Raphael Tripier
Two pyclen based lanthanide chelators, L4band L4c, bearing two specific picolinate 2P antennas
(tailor-made for each targeted metal) and one acetate arm arranged in a
dissymmetrical manner, have been synthesized to form, with the already
described ligand L4a, a
complete family of lanthanide luminescent bioprobes: [EuL4a], [SmL4a],
[YbL4b], [TbL4c] and [DyL4c]. Additionally,
symmetrically arranged regioisomer L4a’was also synthesized as well as its [EuL4a’]
complex to highlight the astonishing positive impact of the dissymmetrical N-distribution of the functional
chelating arm. The investigation clearly shows the high performance of each
bioprobe, which, depending on the complexed lanthanide, could be used in
various applications. Each presents high brightness, quantum yields and lifetimes.
Staining of the complexes into living human breast cancer cells was observed.
In addition, in vivo 2P-microscopy
was performed for the first time on a living Zebrafish model with [EuL4a]. No apparent toxicity
was detected on the growth of the zebrafish and images of high quality were