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COVID19 footprinting manuscript_5.pdf (1.3 MB)

Protein Footprinting, Conformational Dynamics and Interface-Adjacent Neutralization ‘Hotspots’ in the SARS-CoV-2 Spike Protein Receptor Binding Domain / Human ACE2 Interaction

preprint
submitted on 16.12.2020, 09:43 and posted on 18.12.2020, 06:54 by Dominic Narang, Matthew Balmer, D. Andrew James, Derek Wilson
This study provides an HDX-MS based analysis of the interaction between the SARS-CoV-2 spike protein and the human Angiotensin Converting Enzyme 2.

- The data agree exactly with the X-ray co-crystal structure of this complex, but provide additional information based on shifts in dynamics that are observed just outside the interface.

- These dynamic changes occur specifically in regions that are the primary targets of neutralizing antibodies that target spike protein, suggesting that the neutralization mechanism may result from suppression of dynamic shifts in the spike Receptor Binding Domain (RBD) that are necessary for favorable binding thermodynamics in the spike / ACE2 interaction.

Funding

Natural Sciences and Engineering Research Council

Natural Sciences and Engineering Research Council

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History

Email Address of Submitting Author

dkwilson@yorku.ca

Institution

York University

Country

Canada

ORCID For Submitting Author

0000-0002-7012-6085

Declaration of Conflict of Interest

Andrew James and Matthew T. Balmer are employees of Sanofi Pasteur and may hold stock in the company

Version Notes

First Version

Exports