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Protein-Responsive Protein-Release of Supramolecular/polymer Hydrogel Composite Integrating Enzyme Activation Systems

preprint
submitted on 16.01.2020 and posted on 20.01.2020 by Hajime Shigemitsu, Ryou Kubota, Keisuke Nakamura, Tomonobu Matsuzaki, Saori Minami, Kenji Urayama, Itaru Hamachi
Protein-responsive soft materials capable of the controlled release of drugs and proteins are promising for use in next-generation diagnosis methods and therapies. However, it is challenging to rationally design soft materials that respond to non-enzymatic proteins. Here, we describe a supramolecular/agarose hydrogel composite that can release a protein in response to a non-enzymatic protein. A non-enzymatic protein-responsive system was developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set. In situ imaging showed that the supramolecular/agarose hydrogel composite consisted of orthogonal domains of supramolecular fibers and agarose, which played distinct roles in protein entrapment and mechanical stiffness, respectively. Integrating the enzyme activation set with the composite allowed for controlled release of the embedded protein in response to the target protein.

Funding

JSPS KAKENHI Grand Numbers JP17H06348, JP18K14333

JST ERATO Grant Number JPMJER1802

History

Email Address of Submitting Author

ihamachi@sbchem.kyoto-u.ac.jp

Institution

Kyoto University

Country

Japan

ORCID For Submitting Author

0000-0002-3327-3916

Declaration of Conflict of Interest

The authors declare no conflict of interest

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