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Protein-Controlled Actuation of Dynamic Nucleic Acid Networks Using Synthetic DNA Translators

preprint
submitted on 04.06.2020, 14:02 and posted on 05.06.2020, 16:58 by ALESSANDRO BERTUCCI, Alessandro Porchetta, Erica Del Grosso, Tania Patiño, Andrea Idili, Francesco Ricci
Integrating dynamic DNA nanotechnology with protein-controlled actuation will expand our ability to process molecular information. We have developed a strategy to actuate strand displacement reactions using DNA-binding proteins by engineering synthetic DNA translators that convert specific protein-binding events into trigger inputs through a programmed conformational change. We have constructed synthetic DNA networks responsive to two different DNA-binding proteins, TATA-binding protein and Myc-Max, and demonstrated multi-input activation of strand displacement reactions. We finally achieved protein-controlled regulation of a synthetic RNA and of an enzyme through artificial DNA-based communication, showing the potential of our molecular system in performing further programmable tasks.

History

Email Address of Submitting Author

francesco.ricci@uniroma2.it

Institution

University of Rome Tor Vergata

Country

Italia

ORCID For Submitting Author

0000-0003-4941-8646

Declaration of Conflict of Interest

None

Exports