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Langmuir Green Synthesis V7 May 13_Final.pdf (1.64 MB)
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Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

preprint
submitted on 13.05.2020 and posted on 15.05.2020 by Mehvesh Hameed, Seema Panicker, Sallam Hasan Abdallah, Amir A. Khan, Changseok Han, Mohamed Chehimi, Ahmed A. Mohamed
We synthesized protein-coated gold nanoparticles using green and chemical reduction routes for cellular uptake study. In the current work, we coated gold-aryl nanoparticles of the type AuNPs-C6H4-4-COOH with BSA, collagen, zein and lysozyme proteins. Both routes were carried out without phase-transfer catalysts or extraneous stabilizing agents. High crystallinity of the AuNPs synthesized by the green route can be seen in the transmission electron microscopy images. Osteosarcoma cancer cells are malignant bone tumors with abnormal cellular functions. Studies using MG-63 cells will provide mechanistic suggestions on the details of the amplification in tumors. We studied the cellular uptake of the bioconjugates by MG-63 osteosarcoma cells using laser confocal fluorescence microscopy (LCFM) and flow cytometry. In the LCFM study, BSA-AuNPs was uptaken most efficiently of all protein-coated gold nanoparticles synthesized by the green route. Zein and lysozyme coated nanoparticles, though small sizes, prepared by the green method were not efficiently uptaken by MG-63. The two nanoparticles are negatively charged and zein is also a hydrophobic coat. The difference in hydrophobicity and charge might have affected the internalization. All of those coated nanoparticles that were efficiently uptaken can potentially be used as diagnostic and therapeutic agents for osteosarcoma.

Funding

University of Sharjah

Competitive grant 160-2142-029-P

Competitive grant 150-2142-017-P

Competitive grant 160-2145-036-P

Organometallic Research Group grant (RISE-046-2016)

Functionalized Nanomaterials Synthesis Lab grant (151-0039)

History

Email Address of Submitting Author

chehimi@icmpe.cnrs.fr

Institution

CNRS

Country

France

ORCID For Submitting Author

0000-0002-6098-983X

Declaration of Conflict of Interest

The authors declare no conflict of interest

Version Notes

This is the first version to be submitted to Langmuir, ACS.

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