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manuscript-22-10-20.pdf (4.38 MB)
Protect to Detect: A Golgi Apparatus Targeted Probe to Image Mobile Zinc Through the Use of a Lipophilic Cell-Labile Protecting Group Strategy
Preprints are manuscripts made publicly available before they have been submitted for formal peer review and publication. They might contain new research findings or data. Preprints can be a draft or final version of an author's research but must not have been accepted for publication at the time of submission.
The Golgi apparatus requires zinc for its normal function, but the role it plays in these
processes at the sub-cellular level is not well-understood due to the lack of appropriate tools
to image it. Herein, a small molecule Golgi apparatus targeted probe was developed to image
mobile Zn2+. A trityl group was used to protect a Golgi apparatus targeting cysteine residue to
increase membrane permeability, which was then removed in cellulo within 24 hours,
revealing the free cysteine targeting motif to anchor the probe to the Golgi apparatus. The
probe shows good photophysical properties, good selectivity and Zn2+ response over a wide
range of pH as well as low cellular toxicity. The probe was shown to be capable of targeting
the Golgi apparatus and responding to Zn2+ in a number of different cell lines and was also
applied to monitor the change of concentration of mobile Zn2+ in the Golgi apparatus in
response to oxidative stress.