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Probing Cellular Outcomes Using Heterobivalent Constructs

preprint
submitted on 22.01.2019 and posted on 22.01.2019 by Rohit Bhadoria, Kefeng Ping, Christer Lohk, Ivar Järving, Pavel Starkov

Conjugation techniques are central to improving intracellular delivery of bioactive small molecules. However, tracking and assessing the overall biological outcome of these constructs remains poorly understood. We addressed this issue by having developed a focused library of heterobivalent constructs based on Rho kinase inhibitors to probe various scenarios. By comparing induction of a phenotype of interest vs. cell viability vs. cellular uptake, we demonstrate that such conjugates indeed lead to divergent cellular outcomes.

Funding

Estonian Research Council Grant PUT1290

TalTech Young Investigator Grant B62

Institutional Research Funding IUT19–9 of the Estonian Ministry for Education & Research

History

Email Address of Submitting Author

pavel.starkov@taltech.ee

Institution

Tallinn University of Technology

Country

Estonia

ORCID For Submitting Author

0000-0003-1421-4731

Declaration of Conflict of Interest

The authors declare no conflict of interest.

Version Notes

version 1.0

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