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Polymer Microarrays Rapidly Identify Competitive Adsorbents of Virus-like Particles (VLPs)

revised on 20.10.2020, 14:40 and posted on 21.10.2020, 05:06 by Andrew J. Blok, Pratik Gurnani, Alex Xenopoulos, Laurence Burroughs, Joshua Duncan, Richard Urbanowicz, Theocharis Tsoleridis, Helena Müller, Thomas Strecker, Jonathan Ball, Cameron Alexander, Morgan Alexander

The emergence of SARS-CoV-2 highlights the global need for platform technologies to enable rapid development of diagnostics, vaccines, treatments, and personal protective equipment (PPE). However, many current technologies require the detailed mechanistic knowledge of specific material-virion interactions before they can be employed, for example to aid in the purification of vaccine components, or in design of more effective PPE. Here we show that an adaption of polymer micro array method for screening bacterial-surface interactions allows for screening of polymers for desirable material-viron interactions. Non-pathogenic virus like particlesincluding fluorophores are exposed to the arrays in aqueous buffer as a simple model of virons carried to the surface in saliva/sputum. Competitive binding of Lassa and Rubella particles is measured to probe the relative binding properties of a selection of copolymers. This provides the first step in the development of a method for discovery of novel materials with promise for viral binding, with the next being development of this method to assess absolute viral adsorption and assessment of the attenuation of the activity of live virus which we propose would be part of a material scale up step carried out in biological laboratory safety level 4 facilities and the use of more complex media to represent biological fluids.


EMD Millipore Corporation

EPSRC, grant number: EP/R013764/1

Royal Society through a Wolfson Research Merit Award [WM150086]

Engineering and Physical Sciences Research Council [grant number EP/N006615/1]

Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Projektnummer 197785619/SFB1021


Email Address of Submitting Author


University of Nottingham


United Kingdom

ORCID For Submitting Author


Declaration of Conflict of Interest

We declare no conflict of interests

Version Notes

submitted to Biointerphases