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Polymer Microarrays Rapidly Identify Competitive Adsorbents of Virus-like Particles (VLPs)

preprint
submitted on 17.09.2020 and posted on 17.09.2020 by Andrew J. Blok, Pratik Gurnani, Alex Xenopoulos, Laurence Burroughs, Joshua Duncan, Richard Urbanowicz, Theocharis Tsoleridis, Helena Müller, Thomas Strecker, Jonathan Ball, Cameron Alexander, Morgan Alexander
Here we show that an adaption of polymer micro array method for screening bacterial-surface interactions allows for screening of polymers for desirable material-viron interactions. Non-pathogenic virus like particles including fluorophores are exposed to the arrays in aqueous buffer as a simple model of virons carried to the surface in saliva/sputum. Competitive binding of Lassa and Rubella particles is measured to probe the relative binding properties of a selection of copolymers. This provides the first step in the development of a method for discovery of novel materials with promise for viral binding, with the next being development of this method to assess absolute viral adsorption and assessment of the attenuation of the activity of live virus which we propose would be part of a material scale up step carried out in biological laboratory safety level 3 facilities and the use of more complex media to represent biological fluids.

Funding

EMD Millipore Corporation

EPSRC, grant number: EP/R013764/1

Royal Society through a Wolfson Research Merit Award [WM150086]

Engineering and Physical Sciences Research Council [grant number EP/N006615/1]

Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Projektnummer 197785619/SFB1021

History

Email Address of Submitting Author

morgan.alexander@nottingham.ac.uk

Institution

University of Nottingham

Country

United Kingdom

ORCID For Submitting Author

0000-0001-5182-493X

Declaration of Conflict of Interest

We declare no conflict of interests

Version Notes

submitted to Biointerphases

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