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Halabi-PFF2019-finalversion.pdf (18.89 MB)
Photoregulated Fluxional Fluorophores for Live-Cell Super-Resolution Microscopy with No Apparent Photobleaching
Preprints are manuscripts made publicly available before they have been submitted for formal peer review and publication. They might contain new research findings or data. Preprints can be a draft or final version of an author's research but must not have been accepted for publication at the time of submission.
Photoswitchable molecules have found multiple
applications in the physical and life sciences because their properties can be modulated
with light. Fluxional molecules, which undergo rapid degenerate rearrangements
in the electronic ground state, also exhibit switching behavior. The stochastic
nature of fluxional switching, however, has hampered its application in the
development of functional molecules and materials. Here we combine
photoswitching and fluxionality to develop a fluorophore that enables very long
(>30 min) time-lapse single-molecule localization microscopy in living cells
with minimal phototoxicity and no apparent photobleaching. These long
time-lapse experiments allowed us to track intracellular organelles with
unprecedented spatiotemporal resolution, revealing new information of the
three-dimensional compartmentalization of synaptic vesicle trafficking in live