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Photo-Crosslinkable, Insulating Silk Fibroin for Bioelectronics with Enhanced Cell Affinity

preprint
revised on 25.02.2020, 15:02 and posted on 26.02.2020, 06:44 by Jie Ju, Ning Hu, Dana Cairns, Haitao Liu, Brian Timko
Bioelectronic scaffolds that support devices while promoting tissue integration could enable tissue hybrids with augmented electronic capabilities. Here, we demonstrate a Photo-crosslinkable Silk Fibroin (PSF) derivative and investigate its structural, electrical and chemical properties. Lithographically-defined PSF films offered tunable thickness and <1 μm spatial resolution, and could be released from a relief layer yielding freestanding scaffolds with cm-scale uniformity. These constructs were electrically insulating; multi-electrode arrays with PSF-passivated interconnects provided stable electrophysiological readouts from HL-1 cardiac model cells, brain slices and hearts. Compared to SU8, a ubiquitous biomaterial, PSF exhibited superior affinity toward neurons which we attribute to its favorable surface charge and enhanced attachment of poly-d-lysine adhesion factors. This finding is of significant importance in bioelectronics, where tight junctions between devices and cell membranes are necessary for electronic communication. Collectively, our findings are generalizable to a variety of geometries, devices and tissues, establishing PSF as a promising bioelectronic platform.

Funding

Tissue Engineering Resource Center

National Institute of Biomedical Imaging and Bioengineering

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NNCI: The Center for Nanoscale System (CNS) at Harvard University

Directorate for Engineering

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History

Email Address of Submitting Author

brian.timko@tufts.edu

Institution

Tufts University

Country

USA

ORCID For Submitting Author

0000-0002-7958-8980

Declaration of Conflict of Interest

The authors declare no conflicts of interest.

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in Proceedings of the National Academy of Sciences

ChemRxiv

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