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Perchlorinated Triarylmethyl Radical 99% Enriched 13C at the Central Carbon as EPR Spin Probe Highly Sensitive to Molecular Tumbling

preprint
submitted on 27.04.2021, 16:50 and posted on 28.04.2021, 09:26 by Martin Poncelet, Justin L. Huffman, Gareth R. Eaton, Whylder Moore, Sandra Eaton, Benoit Driesschaert

Soluble stable radicals are used as spin probes and spin labels for in vitro and in vivo Electron Paramagnetic Resonance (EPR) spectroscopy and imaging applications. We report the synthesis and characterization of a perchlorinated triarylmethyl radical enriched 99% at the central carbon, 13C1-PTMTC. The anisotropy of the hyperfine splitting with the 13C1 (Ax=26, Ay=25, Az=199.5 MHz) and the g (gx=2.0015, gy=2.0015, gz=2.0040) are responsible for a strong effect of the radical tumbling rate on the EPR spectrum. The rotational correlation time can be determine by spectral simulation or via the linewidth after calibration. As spin probe 13C1-PTMTC can be used to measure media microviscosity with high sensitivity. Bound to a macromolecule as spin label, 13C1-PTMTC could be used to study local mobility and molecular interactions.

Funding

Biocompatible Magnetic Resonance Probes for in vivo Concurrent Profiling

National Institute of Biomedical Imaging and Bioengineering

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Imaging enzyme activity by Overhauser-enhanced MRI

National Institute of Biomedical Imaging and Bioengineering

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Preclinical Electron Paramagnetic Resonance Tumor Imager

National Cancer Institute

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History

Email Address of Submitting Author

benoit.driesschaert@hsc.wvu.edu

Institution

West Virginia University

Country

United States

ORCID For Submitting Author

0000-0002-1402-413X

Declaration of Conflict of Interest

None

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