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Optimal Duration of the Preincubation Phase in Enzyme Inhibition Experiments

preprint
submitted on 22.03.2020 and posted on 23.03.2020 by Petr Kuzmic
This report describes an algebraic formula to calculate the optimal duration of the pre-incubation phase in enzyme-inhibition experiments, based on the assumed range of expected values for the dissociation equilibrium constant of the enzyme–inhibitor complex and for the bimolecular association rate constant. Three typical experimental scenarios are treated, namely, (1) single-point primary screening at relatively high inhibitor concentrations; (2) dose-response secondary screening of relatively weakly bound inhibitors; (3) dose-response screening of tightly-bound inhibitors.

History

Email Address of Submitting Author

petr.kuzmic@gmail.com

Institution

BioKin Ltd.

Country

USA

ORCID For Submitting Author

0000-0001-5250-8381

Declaration of Conflict of Interest

The author declares no conflict of interest.

Version Notes

Initial submission

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