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Online Measurement of Glucose Consumption from HepG2 Cells Using an Integrated Bioreactor and Enzymatic Assay

preprint
submitted on 14.12.2018 and posted on 17.12.2018 by Anna Adams, Radha Krishna Murthy Bulusu, Nikita Mukhitov, Jose Mendoza-Cortes, Michael Roper
In this work, we developed a microfluidic bioreactor for optimizing growth and maintaining structure and function of HepG2, and when desired, the device could be removed and the extracellular output from the bioreactor combined with enzymatic glucose reagents into a droplet-based microfluidic system. The intensity of the resulting fluorescent assay product in the droplets was measured, and was directly correlated to glucose concentration, allowing the effect of insulin on glucose consumption in the HepG2 cells to be observed and quantified online and in near real-time.

Funding

Microfluidic Devices for Determining Dynamics of Islets of Langerhans

National Institute of Diabetes and Digestive and Kidney Diseases

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National High Magnetic Field Laboratory Renewal 2018-2022

Directorate for Mathematical & Physical Sciences

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History

Email Address of Submitting Author

aadams@chem.fsu.edu

Institution

Florida State University

Country

United States of America

ORCID For Submitting Author

0000-0002-2461-2699

Declaration of Conflict of Interest

no conflict of interest

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