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Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells
preprintsubmitted on 29.11.2018, 00:25 and posted on 29.11.2018, 15:32 by Lisa Alcock, Bruno Oliveira, Michael Deery, Tara Pukala, Michael Perkins, Gonçalo Bernardes, Justin Chalker
Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.
Read the published paper
in ACS Chemical Biology