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Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

preprint
submitted on 29.11.2018, 00:25 and posted on 29.11.2018, 15:32 by Lisa Alcock, Bruno Oliveira, Michael Deery, Tara Pukala, Michael Perkins, Gonçalo Bernardes, Justin Chalker
Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

Funding

Australian Research Council (DE150101863)

The Royal Society (UF110046 and URF\R\180019)

FCT Portugal (iFCT, IF/00624/2015)

European Research Council (Starting Grant No. 676832)

Marie Sklodowska-Curie ITN Protein Conjugates (No. 67500)

EPSRC

Australian Government Endeavour Postgraduate Scholarship

History

Email Address of Submitting Author

justin.chalker@flinders.edu.au

Institution

Flinders University

Country

Australia

ORCID For Submitting Author

0000-0002-7504-5508

Declaration of Conflict of Interest

No conflict of interest

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in ACS Chemical Biology

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