ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
five-plex multiplexed NIR-SERRS imaging-Yu JH-ChemRxiv.pdf (3.38 MB)

Noninvasive and Highly Multiplexed Five-Color Tumor Imaging of Near-Infrared Resonant Surface-Enhanced Raman Nanoparticles In Vivo

preprint
submitted on 12.11.2020, 21:43 and posted on 16.11.2020, 07:14 by Jung-Ho Yu, Idan Steinberg, Ryan Miller Davis, Andrey V. Malkovskiy, Aimen Zlitni, Daniel Tan Chung, Luis Dan Curet, Aloma L. D’Souza, Kyung oh Jung, Edwin Chang, Jos Campbell, Hadas Frostig, Seung-min Park, Guillem Pratx, Sanjiv Sam Gambhir
In vivo multiplexed imaging aims for noninvasive analysis of multiple tumor biomarkers. While most of the preclinical imaging has provided a number of multiplexing channels up to three, Raman imaging with surface-enhanced Raman scattering (SERS) nanoparticles was suggested to offer higher multiplexing capability originating from their narrow spectral width. However, the multiplexed Raman imaging is still in its infancy for visualizing tumors with both sufficient multiplicity and high sensitivity concurrently. Here we create multispectral palettes of gold core-near-infrared (NIR) resonant Raman dyes-silica shell SERS (NIR-SERRS) nanoparticles, which provide both the high multiplicity and fluorescence-comparable sensitivity. With the NIR-SERRS nanoparticle palettes, we demonstrate noninvasive and five-plex ratiometric Raman imaging of tumors in living mice. Furthermore, we perform noninvasive and longitudinal imaging of the five-color nanoparticles in the tumors, which is not feasible with current ex vivo multiplexing platforms, demonstrating great potential for noninvasive assessment of multiple biological targets within tumors.

Funding

U54 CA199075

History

Email Address of Submitting Author

junghyu@stanford.edu

Institution

Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford School of Medicine

Country

United States

ORCID For Submitting Author

0000-0003-4101-513X

Declaration of Conflict of Interest

No Conflict of Interest

Exports