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Nongenetic Control of Receptor Signaling Dynamics with a DNA-based Optochemical Tool

preprint
submitted on 07.01.2020, 10:53 and posted on 08.01.2020, 12:58 by Ryosuke Ueki, Shota Hayashi, Masaya Tsunoda, Momoko Akiyama, Hanrui Liu, Tasuku Ueno, Yasuteru Urano, Shinsuke Sando
Optochemical tools that can modulate activity of the target protein provide an opportunity for studying and regulating the related biological processes. Here we present a DNA-based nongenetic optochemical tool that can control dynamics of growth factor signaling. A photo-caged DNA aptamer for a growth factor receptor was designed to assemble into an active dimer form, depending on UV irradiation, thereby enabling optical control of the dimerization and subsequent activation of the receptor. This photo-caged mimicry of growth factor can be a promising tool for elucidating a linkage between dynamics of signaling and resulting biological outcomes, as well as for manipulating cellular functions and the fate of living cells.

Funding

Core Research for Evolutional Science and Technology (CREST) of Molecular Technology (#JPMJCR13L4) from the Japan Science and Technology Agency

Grant-in-Aid for Young Scientists (#19K15693) form the Japan Society for the Promotion of Science

Basic Science Research Projects from the Sumitomo Foundation

Noguchi-Shitagau Research Grant from the Noguchi Institute

History

Email Address of Submitting Author

ssando@chembio.t.u-tokyo.ac.jp

Institution

The University of Tokyo

Country

Japan

ORCID For Submitting Author

0000-0003-0275-7237

Declaration of Conflict of Interest

The authors declare no conflicts of interest associated with this manuscript.

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