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Non-reductive Homolytic Scission of Endoperoxide Bond for Activation of Artemisinin: The Bi-radical Perspectives

preprint
submitted on 23.02.2021, 13:16 and posted on 24.02.2021, 07:32 by Shikha Sharma, Md. Ehesan Ali

Artemisinin is the most famous antimalarial drug against malaria caused by Plasmodium falciparum. Despite its tremendous success and popularity in malaria therapeutics, the molecular mechanism of artemisinin’s activity is still elusive. The activation of artemisinin, i.e., cleavage of the endoperoxide bond at the infected cell that generates radical intermediates and the subsequent chemical rearrangements plays a key role in the antimalarial activities. In this work, applying state-of-the-art computational techniques based on the spin constraint density functional theory (CDFT) along with ab initio thermodynamics, we have investigated various key steps of the molecular mechanism of artemisinin. The well-accepted artemisinin activation process is the reductive heterolytic scission of the endo-peroxide bond which is followed by subsequent chemical reactions that propagate via mono-radical intermediates. Here adopting the CDFT we have investigated the possible alternative ‘biradical’ intermediates and their mechanistic pathways for the subsequent chemical reactions. The change in Gibbs free energy associated with the activation of artemisinin through homolytic-scissoring (biradical) intermediate is quite competitive and favorable compared to the reductive heterolytic-scissoring (monoradical) process. This clearly indicates the alternative possibilities for the biradical activation process. The reported experimental EPR signals for the biradicals especially for similar anti-malarial drugs like G3-factor support our observations.

Funding

SERB-ECR project no. ECR/2016/000362

Indo-Sweden joint project no. DST/INT/SWD/VR/P-01/2016

History

Email Address of Submitting Author

shikha.ph17204@inst.ac.in

Institution

Institute of Nano Science and Technology, Mohali, 140306

Country

India

ORCID For Submitting Author

0000-0001-7966-2899

Declaration of Conflict of Interest

There are no conflicts of interest to declare.

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