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Non-Proteinaceous Complexes III and IV Mimicking Electron Transfer in the Mitochondrial Respiratory Chain

preprint
submitted on 04.04.2020 and posted on 06.04.2020 by Iago Modenez, Lucyano Macedo, Antonio F. A. A. Melo, Andressa R. Pereira, Osvaldo Novais Oliveira Junior, Frank Crespilho

Synthetic biology pursues the understanding of biological processes and their possible mimicry with artificial bioinspired materials. We explore the redox properties of magnetic iron oxide nanoparticles to mimic the redox activity of complexes III and IV towards cytochrome c. We demonstrate that these nanoparticles, incorporated as non-proteinaceous complexes III and IV in a mitochondrial cell membrane model, catalyze electron transfer similarly to natural complexes. The associated molecular mechanism was experimentally proven in solution and in a Langmuir- Blodgett film; the protein-nanoparticle interactions are governed mainly by electrostatic forces, followed by electron transfer between the iron sites of the nanoparticles and the heme group. This work presents the first experimental demonstration that inorganic nanostructured systems may behave as proteins in the cell membrane.

Funding

National Council for Scientific and Technological Development process134396/2018-9

São Paulo Research Foundation process 2017/20493-2

São Paulo Research Foundation process 2018/00878-0

São Paulo Research Foundation process 2017/03879-4

São Paulo Research Foundation process 2019/15333-1

São Paulo Research Foundation process 2019/12053-8

History

Email Address of Submitting Author

iago_modenez@usp.br

Institution

University of São Paulo, São Carlos Institute of Chemistry

Country

Brazil

ORCID For Submitting Author

0000-0003-1470-9013

Declaration of Conflict of Interest

Authors declare no conflict interest.

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