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Non-Equilibrium Generation of Catalytic Supramolecular Polymers of Pre-RNA Nucleobases

preprint
submitted on 12.01.2021, 04:22 and posted on 12.01.2021, 13:03 by Syed Pavel Afrose, Chiranjit Mahato, Pooja Sharma, Lisa Roy, Dibyendu Das

Bioenergetics played critical roles for the chemical emergence of life where available energy resources drove the generation of primitive polymers and fueled early metabolism. Further, apart from information storage, the catalytic roles of primitive nucleic acid fragments have also been argued to be important for biopolymer evolution. Herein, we have demonstrated the non-equilibrium generation of catalytic supramolecular polymers of a possible proto-RNA building block (melamine) driven by a thermodynamically activated ester of low molecular weight. We utilized reversible covalent linkage to install a catalytic imidazole moiety in the polymer backbone. This resulted in energy dissipation via hydrolysis of the substrate predominantly from the assembled state and subsequent disassembly, thus installing kinetic asymmetry in the energy consumption cycle. Non-catalytic analogues led to kinetically stable polymers while inactivated substrates were unable to drive the polymerization. The non-equilibrium polymers of the pre-RNA bases were capable to spatiotemporally bind to a model cofactor. Notably, presence of an exogenous aromatic base augmented the stability of the polymers, reminiscent to what the molecular midwives did during early evolution.

Funding

SERB SJF Grant SB/SJF/2020-21/08

History

Email Address of Submitting Author

dasd@iiserkol.ac.in

Institution

Indian Institute of Science Education and Research (IISER) Kolkata

Country

India

ORCID For Submitting Author

0000-0001-6597-8454

Declaration of Conflict of Interest

Authors declare no conflict of interest

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