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Supercoiling Theory and Model of Chromosomal Structures in Eukaryotic Cells.docx (83.66 MB)
Supercoiling Theory and Model of Chromosomal Structures in Eukaryotic Cells
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billion base pairs of DNA reside highly orderly in each human cell’s nucleus
through their manifestation as twenty-three pairs of chromosomes. Delicate
patterns of spatial organizations of DNA macromolecules in these eukaryotic
chromosomes as well as their associated physical driving forces have, however,
not been fully understood thus far. On the basis
of (1) our four recent discoveries about supercoiling properties of histone H1,
nucleosomes, linker DNA and polynucleosomes, (2) well-accepted six axioms about
signs, shapes and handedness of DNA supercoils, and (3) our three new
prepositions about correlations between DNA supercoils and chromosomal
structures, we formulate new theories and models of eukaryotic chromosomal
structures in the current report. It is our conclusion that all levels of
chromosomal structures in eukaryotic cells are governed mainly by negative
supercoils that are present in their naked linker DNA regions.