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Natural Heterogeneous Catalysis with Immobilised Oxidase Biocatalysts

preprint
submitted on 06.04.2020 and posted on 07.04.2020 by Ashley P. Mattey, Jack J. sangster, Christopher Baldwin, Jeremy I. Ramsden, William R. Birmingham, Rachel S. Heath, antonio angelastro, Nicholas J. Turner, Sebastian Cosgrove, Sabine Flitsch
Characterisation of immobilised oxidase biocatalysts allowing multifunctional oxidation of valuable chemicals is described. Engineered galactose oxidase (GOase) variants M1 and M3-5, an engineered choline oxidase (AcCO6) and monoamine oxidase (MAO-N D9) displayed long-term stability and reusability over several weeks when covalently attached on solid support, outperforming their free counterparts in terms of stability, resistance to heat, and tolerance to neat organic solvents. While immobilisation of oxidase biocatalysts improves properties that are critical for industrial implementation, they additionally showed versatility as the biocatalyst batches can be recovered, washed and reused multiple times for the oxidation of different substrates.

Funding

EP/S01778X/1

BB/L013762/1

BB/M027791/1

BB/M02903411

BB/M028836/1

788231-ProgrES-ERC-2017-ADG

History

Email Address of Submitting Author

sebastian.cosgrove@manchester.ac.uk

Institution

University of Manchester

Country

United Kingdom

ORCID For Submitting Author

0000-0001-9541-7201

Declaration of Conflict of Interest

No conflict of interest.

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