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submitted on 06.04.2020 and posted on 07.04.2020by Ashley P. Mattey, Jack J. sangster, Christopher Baldwin, Jeremy I. Ramsden, William R. Birmingham, Rachel S. Heath, antonio angelastro, Nicholas J. Turner, Sebastian Cosgrove, Sabine Flitsch
Characterisation of immobilised oxidase biocatalysts allowing
multifunctional oxidation of valuable chemicals is described.
Engineered galactose oxidase (GOase) variants M1 and M3-5, an
engineered choline oxidase (AcCO6) and monoamine oxidase
(MAO-N D9) displayed long-term stability and reusability over
several weeks when covalently attached on solid support,
outperforming their free counterparts in terms of stability,
resistance to heat, and tolerance to neat organic solvents. While
immobilisation of oxidase biocatalysts improves properties that are
critical for industrial implementation, they additionally showed
versatility as the biocatalyst batches can be recovered, washed and
reused multiple times for the oxidation of different substrates.