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LH-LiGluR-Garrido-Charles2019.pdf (3.65 MB)
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Nanoengineered Light-Harvested Proteins for Optogenetics and Photopharmacology

preprint
submitted on 21.12.2019 and posted on 23.12.2019 by Aida Garrido-Charles, Gisela Cabré, Marta Gascón-Moya, Félix Busqué, Ramon Alibés, Jordi Hernando, Pau Gorostiza

Chemical modification with nanometer precision can be used to probe and to improve the function of complex molecular entities, from organic materials to proteins and their assemblies. Using the pigment arrangement in photosynthetic light-harvesting as inspiration, we show that molecular photosensitizers can be located at well-defined distances from photoisomerizable units in proteins in order to enhance and spectrally shift their photoresponses. The approach is demonstrated in Channelrhodopsin-2 (ChR2) and in the light-gated ionotropic glutamate receptor (LiGluR), two archetypical actuators in optogenetics and photopharmacology that have been used both for fundamental and therapeutic purposes. These proof-of-concept experiments together with theoretical simulations predict that the photosensitivity can be increased several orders of magnitude using these means, thus providing a unique methodology to boost the performance of current optogenetic and photopharmacological toolboxes.

Funding

CTQ2015-65439-R

CTQ2016-80066-R

CTQ2016-75363-R

2017-SGR-00465

2017-SGR-1442

Human Brain Project WAVESCALES projects

Fundaluce foundation

BES-2014-068169

History

Email Address of Submitting Author

agarrido@ibecbarcelona.eu

Institution

IBEC

Country

Spain

ORCID For Submitting Author

0000-0002-9577-6682

Declaration of Conflict of Interest

We declare no conflict of interest.

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