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NMR Resolution Enhancement and Homonuclear Decoupling Using Non-Uniform Weighted Sampling

submitted on 19.03.2020, 17:16 and posted on 20.03.2020, 09:46 by Chris Waudby, John Christodoulou
Non-uniform weighted sampling (NUWS) is a simple method for multi-dimensional NMR spectroscopy in which window functions are applied during acquisition by sampling varying numbers of scans across indirect dimensions. While NUWS was previously shown to provide modest increases in sensitivity, here we describe a complementary application to enhance spectral resolution by increasing the sampling of later points of the time domain signal. Moreover, by combining NUWS with carefully constructed apodization functions signal envelopes can be modulated in an arbitrary manner while retaining a uniform noise level, permitting further signal manipulations such as linear prediction and non-uniform sampling (NUS). We leverage this to develop a combined NUWS-NUS scheme for broadband homonuclear decoupling, with substantially increased sensitivity in comparison to constant time experiments.


Integrative structural biology of protein folding during biosynthesis on the ribosome

Wellcome Trust

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BBSRC BB/T002603/1

NMR Centre

Cancer Research UK

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University College London



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