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Mycobacterium Survival Strategy Translated to Develop a Lipo-Peptide Based Fusion Inhibitor

preprint
submitted on 05.05.2020 and posted on 07.05.2020 by Avijit Sardar, Aritraa Lahiri, Amirul Islam Mallick, Pradip Kumar Tarafdar
The entry of enveloped viruses requires fusion of viral and host cell membranes. An effective fusion inhibitor aiming at impeding such virus-host cell membrane fusion may emerge as a broad-spectrum antiviral agent to neutralize the infection from an increasing diversity of harmful new viruses. Mycobacterium survives inside the phagosome of the host cells by inhibiting phagosome-lysosome fusion with the help of a coat protein coronin 1. Structural analysis of coronin 1 and other WD40-repeat containing protein suggest that the tryptophan-aspartic acid (WD) sequence is placed at distorted β-meander motif (more exposed) whereas the WD resides in regular β-meander motif in other WD40 proteins. The unique structural feature of coronin 1 was explored to identify a simple lipo-peptide sequence (lipid-WD), which effectively inhibit the membrane fusion by increasing interfacial order and decreasing water penetration, surface potential. The effective fusion inhibitory role of mycobacterium inspired lipo-dipeptide was applied to combat type 1 influenza virus (H1N1) infection as a ‘broad spectrum’ antiviral agent.

Funding

DST-Inspire (IFA13-CH-120, 2014)

Early Career Award (ECR/2016/001935)

History

Email Address of Submitting Author

srpradip@gmail.com

Institution

Indian Institute of Science Education and Research Kolkata

Country

India

ORCID For Submitting Author

0000-0002-1059-950X

Declaration of Conflict of Interest

No

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