These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
Qian et al manuscript.pdf (7.29 MB)

Multivalent Cluster Nanomolecules for Inhibiting Protein-Protein Interactions

submitted on 23.07.2019, 03:05 and posted on 23.07.2019, 15:46 by Elaine A. Qian, Yanxiao Han, Marco Messina, Heather D. Maynard, Petr Král, Alexander Spokoyny
Multivalent protein-protein interactions serve central roles in many essential biological processes, ranging from cell signaling and adhesion to pathogen recognition. Uncovering the rules that govern these intricate interactions is important not only to basic biology and chemistry, but also to the applied sciences where researchers are interested in developing molecules to promote or inhibit these interactions. Here we report the synthesis and application of atomically precise inorganic cluster nanomolecules consisting of an inorganic core and a covalently linked densely packed layer of saccharides. These hybrid agents are stable under biologically relevant conditions and exhibit multivalent binding capabilities, which enable us to study the complex interactions between glycosylated structures and a dendritic cell lectin receptor. Importantly, we find that subtle changes in the molecular structure lead to significant differences in the nanomolecule’s protein binding properties. Furthermore, we demonstrate an example of using these hybrid nanomolecules to effectively inhibit protein-protein interactions in a human cell line. Ultimately, this work reveals an intricate interplay between the structural design of multivalent agents and their biological activities toward protein surfaces.


Email Address of Submitting Author


University of California, Los Angeles



ORCID For Submitting Author


Declaration of Conflict of Interest

Authors declare no conflict of interests.