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Multiplexed Online-Monitoring of Microfluidic Free-Flow Electrophoresis via Mass Spectrometry

preprint
revised on 19.02.2020 and posted on 20.02.2020 by Matthias Jender, Pedro Novo, Dominic Maehler, Ute Münchberg, Dirk Janasek, Erik Freier
Free-flow electrophoresis is a tool for the continuous fractionation of electrically charged analytes. In this study we introduce a novel method to couple microchip-based free-flow electrophoresis with mass spectrometry. The successive connection of multiple microchip outlets to the electrospray ionization source of a mass spectrometer is automated using a multiposition valve. With this novel setup it is possible to continuously fractionate and collect compounds while simultaneously monitoring the process online with mass spectrometry. The functionality of the method is demonstrated by the successful separation and identification of the biomolecules AMP, ATP and CoA, which are fundamental for numerous biochemical processes in every organism.

Funding

Biotechnology2020 + Structural Projects: Leibniz Research Cluster (LRC) - Bio / Synthetic Multifunctional Micro-Production Units - Novel Pathways to Drug Development, Subproject E

Federal Ministry of Education and Research

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Ministerium für Kultur und Wissenschaft des Landes Nordrhein-Westfalen

Bundesministerium für Bildung und Forschung

History

Email Address of Submitting Author

erik.freier@isas.de

Institution

Leibniz-Institut für Analytische Wissenschaften -ISAS- e.V.

Country

Germany

ORCID For Submitting Author

0000-0002-0559-4210

Declaration of Conflict of Interest

no conflict of interest

Version Notes

Corrected typo in Fig. 4 and designation of funding body

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