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Final Manuscript IF-3.pdf (1.41 MB)

Structural and Functional Elucidation of IF-3 Protein of Chloroflexus aurantiacus Involved in Protein Biosynthesis: An In-Silico Approach

revised on 11.04.2021, 18:11 and posted on 12.04.2021, 09:22 by Abu Saim Mohammad Saikat, Md. Ekhlas Uddin, Tasnim Ahmad, Shahriar Mahmud, Md. Abu Sayeed Imran, Sohel Ahmed, Salem A. Alyami, Mohammad Ali Moni

Chloroflexus aurantiacus is a thermophilic bacterium that produces a multitude of proteins
within its genome. Bioinformatics strategies can facilitate comprehending this organism through
functional and structural interpretation assessments.This study aimed to allocate the structure and
function through an in-silico approach required for bacterial protein biosynthesis. This in-silico
viewpoint provides copious properties, including the physicochemical properties, subcellular location,
three-dimensional structure, protein-protein interactions, and functional elucidation of the protein
(WP_012256288.1). The STRING program is utilized for the explication of protein-protein
interactions. The in-silico investigation documented the protein's hydrophilic nature with
predominantly alpha (α) helices in its secondary structure.The tertiary-structure model of the protein
has been shown to exhibit reasonably high consistency based on various quality assessment
methods.The functional interpretation suggested that the protein can act as a translation initiation
factor, a protein required for translation and protein biosynthesis. Protein-protein interactions also
demonstrated high credence that the protein interconnected with 30S ribosomal subunit involved in
protein synthesis. This study is bioinformatically examined that the protein (WP_012256288.1) is
affiliated in protein biosynthesis as a translation initiation factor IF-3 of C. aurantiacus.


Email Address of Submitting Author


Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj



ORCID For Submitting Author


Declaration of Conflict of Interest

The authors have no conflicts of interest to declare.

Version Notes

Manuscript data has been updated.