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Final Manuscript IF-3.pdf (1.41 MB)

Structural and Functional Elucidation of IF-3 Protein of Chloroflexus aurantiacus Involved in Protein Biosynthesis: An In-Silico Approach

preprint
revised on 11.04.2021, 18:11 and posted on 12.04.2021, 09:22 by Abu Saim Mohammad Saikat, Md. Ekhlas Uddin, Tasnim Ahmad, Shahriar Mahmud, Md. Abu Sayeed Imran, Sohel Ahmed, Salem A. Alyami, Mohammad Ali Moni

Chloroflexus aurantiacus is a thermophilic bacterium that produces a multitude of proteins
within its genome. Bioinformatics strategies can facilitate comprehending this organism through
functional and structural interpretation assessments.This study aimed to allocate the structure and
function through an in-silico approach required for bacterial protein biosynthesis. This in-silico
viewpoint provides copious properties, including the physicochemical properties, subcellular location,
three-dimensional structure, protein-protein interactions, and functional elucidation of the protein
(WP_012256288.1). The STRING program is utilized for the explication of protein-protein
interactions. The in-silico investigation documented the protein's hydrophilic nature with
predominantly alpha (α) helices in its secondary structure.The tertiary-structure model of the protein
has been shown to exhibit reasonably high consistency based on various quality assessment
methods.The functional interpretation suggested that the protein can act as a translation initiation
factor, a protein required for translation and protein biosynthesis. Protein-protein interactions also
demonstrated high credence that the protein interconnected with 30S ribosomal subunit involved in
protein synthesis. This study is bioinformatically examined that the protein (WP_012256288.1) is
affiliated in protein biosynthesis as a translation initiation factor IF-3 of C. aurantiacus.

History

Email Address of Submitting Author

asmsaikat.bmb@gmail.com

Institution

Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj

Country

Bangladesh

ORCID For Submitting Author

0000-0002-6850-1245

Declaration of Conflict of Interest

The authors have no conflicts of interest to declare.

Version Notes

Manuscript data has been updated.

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