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submitted on 02.04.2020 and posted on 03.04.2020by Nikola Lončar, Henriette J. Rozeboom, Linda E. Franken, Marc C. A. Stuart, Marco Fraaije
In this paper, we report on the discovery of a
novel, robust protein cage (encapsulin) that we could use for packaging various
biocatalysts. We have elucidated the structure of the stable encapsulin by
electron microscopy and X-ray diffraction. Furthermore, we developed an effective expression system for the encapsulin and a facile protocol for preparing encapsulated enzymes. By packaging and testing
various enzymes (varying in size, oligomeric structure, and cofactor type) we
demonstrate that, through encapsulation, the enzymes become significantly more
stable. We also provide evidence that the pores of the encapsulin, through a
size-exclusion effect, can modulate the substrate acceptance profile of an