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Molecular-Level Interactions of Nanodisc-Forming Copolymers Dissected by EPR Spectroscopy

submitted on 23.01.2021, 12:09 and posted on 25.01.2021, 13:37 by Jana Eisermann, Matthias Hoffmann, Florian A. Schöffmann, manabendra das, Carolyn Vargas, Sandro Keller, Dariush Hinderberger
In this study, we focus on analyzing the non-covalent interaction patterns between three lipid-nanodisc-forming polymers and nitroxide radicals which are used as small organic tracer molecules. Besides the negatively charged polymers DIBMA and SMA(2:1), we characterize the solvation behavior of a new zwitterionic styrene/maleic amide sulfobetaine copolymer named SMA-SB. The used nitroxide spin probes vary in their respective chemical structure, allowing the report of different local micropolarities and nanoscopic regions by recording temperature-dependent continuous-wave electron paramagnetic resonance (CW EPR) spectra. In combination with dynamic light scattering (DLS) and electrophoretic light scattering (ELS) experiments, we are able to provide a nanoscopic interpretation of the dominant interaction patterns between the polymer and the chosen guest molecule.


Alexander von Humboldt Foundation

Fonds der Chemischen Industrie (FCI)

Carl Zeiss Stiftung, Centre for Lipidomics (CZSLip)

Deutsche Forschungsgemeinschaft (DFG), RTG2670 "Beyond Amphphilicity"


Email Address of Submitting Author


Martin Luther University Halle-Wittenberg, Institute of Chemistry



ORCID For Submitting Author


Declaration of Conflict of Interest

The authors declare noc conflicts of interest exist.

Version Notes

V 1.0, 23 January 2021