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Rath_2DPN_v10_chemrxiv.pdf (4.08 MB)

Modelling-Guided Design of Paper Microfluidic Networks – A Case Study of Sequential Fluid Delivery

submitted on 23.07.2020, 10:26 and posted on 23.07.2020, 13:27 by Dharitri Rath, Bhushan Toley

Paper-based microfluidic devices are popular for their ability to automate multi-step assays for chemical or biological sensing at a low cost, but the design of paper microfluidic networks has largely relied on experimental trial and error. A few mathematical models of flow through paper microfluidic devices have been developed and have succeeded in explaining experimental flow behaviour. However, the reverse engineering problem of designing complex paper networks guided by appropriate mathematical models is largely unsolved. In this article, we demonstrate that a two-dimensional paper network (2DPN) designed to sequentially deliver three fluids to a test zone on the device can be computationally designed and experimentally implemented without trial and error. This was accomplished by three new developments in modelling flow through paper networks: i) coupling of the Richards equation of flow through porous media to the species transport equation, ii) modelling flow through assemblies of multiple paper materials (test membrane and wicking pad), and iii) incorporating limited-volume fluid sources. We demonstrate the application of this model in the optimal design of a paper-based signal-enhanced immunoassay for a malaria protein, PfHRP2. This work lays the foundation for the development of a computational design toolbox to aid in the design of paper microfluidic networks.


Extramural Research Grant (EMR/2016/006029) from Science and Engineering Research Board, India

National Postdoctoral Fellowship (PDF/2017/002181) from Science and Engineering Research Board, India


Email Address of Submitting Author


Indian Institute of Science



ORCID For Submitting Author


Declaration of Conflict of Interest