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Modelling-Guided Design of Paper Microfluidic Networks – A Case Study of Sequential Fluid Delivery

preprint
submitted on 23.07.2020 and posted on 23.07.2020 by Dharitri Rath, Bhushan Toley

Paper-based microfluidic devices are popular for their ability to automate multi-step assays for chemical or biological sensing at a low cost, but the design of paper microfluidic networks has largely relied on experimental trial and error. A few mathematical models of flow through paper microfluidic devices have been developed and have succeeded in explaining experimental flow behaviour. However, the reverse engineering problem of designing complex paper networks guided by appropriate mathematical models is largely unsolved. In this article, we demonstrate that a two-dimensional paper network (2DPN) designed to sequentially deliver three fluids to a test zone on the device can be computationally designed and experimentally implemented without trial and error. This was accomplished by three new developments in modelling flow through paper networks: i) coupling of the Richards equation of flow through porous media to the species transport equation, ii) modelling flow through assemblies of multiple paper materials (test membrane and wicking pad), and iii) incorporating limited-volume fluid sources. We demonstrate the application of this model in the optimal design of a paper-based signal-enhanced immunoassay for a malaria protein, PfHRP2. This work lays the foundation for the development of a computational design toolbox to aid in the design of paper microfluidic networks.

Funding

Extramural Research Grant (EMR/2016/006029) from Science and Engineering Research Board, India

National Postdoctoral Fellowship (PDF/2017/002181) from Science and Engineering Research Board, India

History

Email Address of Submitting Author

bhushan@iisc.ac.in

Institution

Indian Institute of Science

Country

India

ORCID For Submitting Author

0000-0003-0119-2350

Declaration of Conflict of Interest

None

Exports