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Microfluidic Microbioreactor to Reduce the Cost and Speed Up Optimisation of Protein Production
preprintsubmitted on 29.02.2020, 23:18 and posted on 03.03.2020, 05:55 by Mayur Parekh, abdulaziz ali, Simon Bateson, Fathi Abugchem, Leon Pybus, Christopher Lennon, Zulfiqur Ali
Here, we demonstrate a low-cost polymer microfluidic microbioreactor with a working volume of 1 mL, integrated with optical sensors for pH, oxygen and cell density, and maintained at constant set temperature for the optimisation of recombinant protein production from Pichia pastoris. Oxygen is transferred from a headspace enclosure, formed using inkjet 3D printing, through a gas-permeable membrane within the microbioreactor with a KLa of 90 at 1500 rpm. A pressurised fluid driving system is used with flow rates controllable to 0.7 µL/min with fluid switching from four reservoirs performed off the microfluidic microbioreactor element so that this can be produced at low cost using high replication techniques.