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submitted on 29.02.2020 and posted on 03.03.2020by Mayur Parekh, abdulaziz ali, Simon Bateson, Fathi Abugchem, Leon Pybus, Christopher Lennon, Zulfiqur Ali
Here, we demonstrate a low-cost polymer microfluidic microbioreactor
with a working volume of 1 mL, integrated with optical sensors for pH, oxygen
and cell density, and maintained at constant set temperature for the
optimisation of recombinant protein production from Pichia pastoris. Oxygen is transferred from a headspace enclosure,
formed using inkjet 3D printing, through
a gas-permeable membrane within the microbioreactor with a KLaof
90 at 1500 rpm. A pressurised fluid driving system is used with
flow rates controllable to 0.7 µL/min with fluid switching from four reservoirs performed off the microfluidic
microbioreactor element so that this can be produced at low cost using high