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Metabolic Characterisation of Magnetospirillum Gryphiswaldense MSR-1 Using LC-MS-Based Metabolite Profiling

preprint
submitted on 17.06.2020, 14:32 and posted on 18.06.2020, 12:14 by Salah Abdelrazig, Laudina Safo, Graham A Rance, Michael W Fay, Eirini Theodosiou, Paul D Topham, Dong-Hyun Kim, Alfred Fernandez-Castane

Magnetosomes are nano-sized magnetic nanoparticles with exquisite properties that can be used in a wide range of healthcare and biotechnological applications. They are biosynthesised by magnetotactic bacteria (MTB) such as Magnetospirillum gryphiswaldense MSR-1 (Mgryph). However, magnetosome bioprocessing yields low quantities compared to chemical synthesis of magnetic nanoparticles. Therefore, the understanding of the intracellular metabolites and the metabolic networks related to Mgryph growth and magnetosome formation are vital to unlock the potential of this organism to develop improved bioprocesses. In this work, we investigated the metabolism of Mgryph using untargeted metabolomics. Liquid chromatography-mass spectrometry (LC-MS) was performed to profile spent medium samples of Mgryph cells grown under O2-limited (n=6) and O2-rich conditions (n=6) corresponding to magnetosome- and non-magnetosome producing cells, respectively. Cross-validated multivariate, univariate and pathway enrichment analyses were conducted to identify significantly altered metabolites and pathways. Rigorous metabolite identification was carried out using authentic standards, Mgryph-specific metabolite database and MS/MS mzCloud database. PCA and OPLS-DA showed clear separation and clustering of sample groups with cross-validation values of R2X=0.76, R2Y=0.99 and Q2=0.98 in OPLS-DA. As a result, 50 metabolites linked to 45 metabolic pathways were found significantly altered in the tested conditions including glycine, serine and threonine; butanoate; alanine, aspartate and glutamate metabolism; aminoacyl-tRNA biosynthesis and; pyruvate and citric acid cycle (TCA) metabolisms. Our findings demonstrate the potential of LC-MS to characterise key metabolites in Mgryph and will contribute to further understand the metabolic mechanisms that affect Mgryph growth and magnetosome formation.

History

Email Address of Submitting Author

a.fernandez-castane1@aston.ac.uk

Institution

Aston University

Country

United Kingdom

ORCID For Submitting Author

0000-0002-2572-7797

Declaration of Conflict of Interest

The authors declare no competing financial interest.

Version Notes

As submitted to RSC Advances

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