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Vercruysse_Melanogenesis involving tyrosinate.pdf (2.7 MB)
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Melanogenesis Using Tyrosinate (Not Tyrosinase)

preprint
submitted on 06.08.2018 and posted on 07.08.2018 by Koen Vercruysse, Nahfisa Richardson

We present our initial observations regarding the effect of the presence of L-tyrosinate (= L-tyrosine disodium salt) on the auto- or Fe2+/H2O2-mediated oxidation of various catecholic substances into melanin-like pigments. We observed that L-tyrosinate inhibited the Fe2+/H2O2-mediated oxidation. In contrast, L-tyrosinate promoted the auto-oxidation of ortho-diphenols like L-DOPA, dopamine, epinephrine, norepinephrine, catechol or pyrogallol, but not a meta-diphenol like resorcinol. In addition, we briefly demonstrated the melanogenic properties of cell culture media containing L-tyrosinate. The reactions were monitored using UV-Vis spectroscopy and size exclusion chromatography. For a reaction between L-tyrosinate and L-DOPA, a large scale experiment was set up allowing us to isolate, purify and characterize using FT-IR spectroscopy the melanin-like material obtained. We discuss our observations in the context of the in vitro and in vivo study of melanogenesis and provide some directions for future research efforts.

History

Email Address of Submitting Author

kvercruysse@tnstate.edu

Institution

Tennessee State University

Country

USA

ORCID For Submitting Author

0000-0003-2420-1985

Declaration of Conflict of Interest

There are no conflicts of interest for both authors.

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