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Mechanoenzymatic Breakdown of Chitinous Material to N-Acetylglucosamine: The Benefits of a Solvent-Free Environment

preprint
submitted on 04.05.2019 and posted on 06.05.2019 by J. P. Daniel Therien, Fabien Hammerer, Tomislav Friscic, Karine Auclair
We report an innovative and efficient mechanoenzymatic method to hydrolyze chitin to N-acetylglucosamine monomer using chitinase under the recently developed RAging methodology, i.e. a combination of mechanochemical ball milling activation and accelerated aging repeated over many cycles, in the absence of bulk solvent. Our result show that the activity of chitinases increases several times by switching from traditional solvent-based conditions of enzymatic catalysis to solvent-free RAging, which operates on moist solid substrates. Importantly, RAging is also highly efficient for the production of N-actylglucosamine directly from shrimp and crab shell biomass without any other processing, except for a gentle wash with aqueous acetic acid.

Funding

GreenCentre Canada G16-121 X-247963

FRQNT Team Grant PR-254169

History

Email Address of Submitting Author

tomislav.friscic@mcgill.ca

Institution

McGill University

Country

Canada

ORCID For Submitting Author

0000-0002-3921-7915

Declaration of Conflict of Interest

Some of the herein presented work is a part of the patent application US 62/465,443 filed 1 Mar 2017.

Version Notes

This is version 1.0

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