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submitted on 04.05.2019 and posted on 06.05.2019by J. P. Daniel Therien, Fabien Hammerer, Tomislav Friscic, Karine Auclair
report an innovative and efficient mechanoenzymatic method to hydrolyze
chitin to N-acetylglucosamine monomer using chitinase under the
recently developed RAging methodology, i.e. a combination of
mechanochemical ball milling activation and accelerated aging repeated
over many cycles, in the absence of bulk solvent. Our result show that
the activity of chitinases increases several times by switching from
traditional solvent-based conditions of enzymatic catalysis to
solvent-free RAging, which operates on moist solid substrates.
Importantly, RAging is also highly efficient for the production of
N-actylglucosamine directly from shrimp and crab shell biomass without
any other processing, except for a gentle wash with aqueous acetic acid.